Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # Evan

Format

BSA Free

Concentration

0.5 mg/ml

View all GATA-4 Antibodies »

Product Summary for GATA-4 Antibody (Evan) - BSA Free

Immunogen

The immunogen for this antibody was Gata4.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for GATA-4 Antibody (Evan) - BSA Free

Western Blot: GATA-4 Antibody (Evan)BSA Free [NBP1-43112]

Western Blot: GATA-4 Antibody (Evan)BSA Free [NBP1-43112]

Western Blot: GATA-4 Antibody (Evan) [NBP1-43112] - Anti-Human/Mouse Gata-4 Purified (2 ug/ml) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart.

Applications for GATA-4 Antibody (Evan) - BSA Free

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
This Evan antibody has been tested by western blot analysis of mouse heart lysates. As a starting dilution, it is recommended to use this purified antibody at 2ug/ml for western blot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GATA-4

The Evan monoclonal antibody reacts with mouse and human Gata-4. In a western blot of C57Bl/6 and BALB/c mouse heart lysates, a single band is present, suggesting that this antibody does not cross-react with Gata-5 or Gata-6, which are also expressed in the heart, but with different molecular weights. The Evan monoclonal antibody has been successfully used in flow cytometry on HEK 293T cells transiently transfected with either mouse Gata-4 or human Gata-4. Gata-4 is a member of the GATA family of transcription factors, which are characterized by a conserved DNA binding domain containing two zinc fingers. The mouse Gata-4 gene encodes a protein of 48kDa. Studies show that Gata-4 plays an important role in controlling cardiomyocyte differentiation, proliferation and survival. Gata-4 is expressed during fetal development, as well as in the adult mouse heart. It also appears to modulate gene expression in the gut, liver, and gonads.

Alternate Names

GATA4

Gene Symbol

GATA4

Additional GATA-4 Products

Product Documents for GATA-4 Antibody (Evan) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GATA-4 Antibody (Evan) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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