GATA-4 Antibody (OTI9F9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70797

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI9F9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GATA-4 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GATA-4 Antibody (OTI9F9) - Azide and BSA Free

Western Blot: GATA-4 Antibody (OTI9F9)Azide and BSA Free [NBP2-70797]

Western Blot: GATA-4 Antibody (OTI9F9)Azide and BSA Free [NBP2-70797]

Western Blot: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA4.
Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797]

Immunocytochemistry/ Immunofluorescence: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797]

Immunocytochemistry/Immunofluorescence: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4.
Immunohistochemistry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797]

Immunohistochemistry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797]

Immunohistochemistry: GATA-4 Antibody (OTI9F9) - Azide and BSA Free [NBP2-70797] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GATA4 mouse monoclonal antibody.
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Applications for GATA-4 Antibody (OTI9F9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GATA-4

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Alternate Names

GATA4

Gene Symbol

GATA4

Additional GATA-4 Products

Product Documents for GATA-4 Antibody (OTI9F9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GATA-4 Antibody (OTI9F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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