GCAT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81564

Novus Biologicals, part of Bio-Techne
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NBP1-81564
NBP1-81564-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCAT Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCAT Antibody

Immunocytochemistry/ Immunofluorescence: GCAT Antibody [NBP1-81564]

Immunocytochemistry/ Immunofluorescence: GCAT Antibody [NBP1-81564]

Immunocytochemistry/Immunofluorescence: GCAT Antibody [NBP1-81564] - Staining of human cell line U-2 OS shows localization to nuclear speckles & mitochondria.
Immunohistochemistry: GCAT Antibody [NBP1-81564]

Immunohistochemistry: GCAT Antibody [NBP1-81564]

Immunohistochemistry: GCAT Antibody [NBP1-81564] - Staining of human pancreas shows moderate cytoplasmic positivity in with a granular pattern in exocrine and endocrine cells.

Applications for GCAT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCAT

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threoninedehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrateby L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reactionbetween 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a classII pyridoxal-phosphate-dependent aminotransferase. (provided by RefSeq)

Alternate Names

2-amino-3-ketobutyrate-CoA ligase, AKB ligase, Aminoacetone synthase, EC 2.3.1, EC 2.3.1.29, Glycine acetyltransferase, glycine C-acetyltransferase, glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase), KBL2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, MGC23053

Gene Symbol

GCAT

Additional GCAT Products

Product Documents for GCAT Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GCAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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