GCAT Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-04780

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-04780-100ul
NBP3-04780-20ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GCAT Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 170-419 of human GCAT (NP_055106.1). KAHKYRYRHLDMADLEAKLQEAQKHRLRLVATDGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINSTLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQSMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVVPKGKARIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCAT Antibody - Azide and BSA Free

Western Blot: GCAT AntibodyAzide and BSA Free [NBP3-04780]

Western Blot: GCAT AntibodyAzide and BSA Free [NBP3-04780]

Western Blot: GCAT Antibody [NBP3-04780] - Analysis of extracts of mouse eye, using GCAT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit .
Immunocytochemistry/ Immunofluorescence: GCAT Antibody - Azide and BSA Free [NBP3-04780]

Immunocytochemistry/ Immunofluorescence: GCAT Antibody - Azide and BSA Free [NBP3-04780]

Immunocytochemistry/Immunofluorescence: GCAT Antibody [NBP3-04780] - Analysis of C6 cells using GCAT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: GCAT Antibody - Azide and BSA Free [NBP3-04780]

Immunohistochemistry-Paraffin: GCAT Antibody - Azide and BSA Free [NBP3-04780]

Immunohistochemistry-Paraffin: GCAT Antibody [NBP3-04780] - Paraffin-embedded human colon carcinoma using GCAT antibody at dilution of 1:100 (40x lens).

Applications for GCAT Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCAT

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threoninedehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrateby L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reactionbetween 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a classII pyridoxal-phosphate-dependent aminotransferase. (provided by RefSeq)

Alternate Names

2-amino-3-ketobutyrate-CoA ligase, AKB ligase, Aminoacetone synthase, EC 2.3.1, EC 2.3.1.29, Glycine acetyltransferase, glycine C-acetyltransferase, glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase), KBL2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, MGC23053

Gene Symbol

GCAT

Additional GCAT Products

Product Documents for GCAT Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCAT Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.