GCKR Antibody (OTI1E7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70823

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GCKR Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GCKR (NP_001417) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCKR Antibody (OTI1E7) - Azide and BSA Free

Western Blot: GCKR Antibody (OTI1E7)Azide and BSA Free [NBP2-70823]

Western Blot: GCKR Antibody (OTI1E7)Azide and BSA Free [NBP2-70823]

Western Blot: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCKR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCKR.
Immunocytochemistry/ Immunofluorescence: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823]

Immunocytochemistry/ Immunofluorescence: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823]

Immunocytochemistry/Immunofluorescence: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCKR.
Immunohistochemistry: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823]

Immunohistochemistry: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823]

Immunohistochemistry: GCKR Antibody (OTI1E7) - Azide and BSA Free [NBP2-70823] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GCKR mouse monoclonal antibody.
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Applications for GCKR Antibody (OTI1E7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GCKR

GCKR encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq]

Alternate Names

FGQTL5, GKRP, glucokinase (hexokinase 4) regulator, glucokinase (hexokinase 4) regulatory protein, Glucokinase regulator, glucokinase regulatory protein

Gene Symbol

GCKR

Additional GCKR Products

Product Documents for GCKR Antibody (OTI1E7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GCKR Antibody (OTI1E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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