GCLC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81840

Novus Biologicals, part of Bio-Techne
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NBP1-81840
NBP1-81840-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCLC Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCLC Antibody

Western Blot: GCLC Antibody [NBP1-81840]

Western Blot: GCLC Antibody [NBP1-81840]

Western Blot: GCLC Antibody [NBP1-81840] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: GCLC Antibody [NBP1-81840]

Immunocytochemistry/ Immunofluorescence: GCLC Antibody [NBP1-81840]

Immunocytochemistry/Immunofluorescence: GCLC Antibody [NBP1-81840] - Staining of human cell line A-431 shows positivity in nucleus.
Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840]

Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840]

Immunohistochemistry-Paraffin: GCLC Antibody [NBP1-81840] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
View 3 more images

Applications for GCLC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Long Name

Glutamate--cysteine ligase catalytic subunit

Alternate Names

EC 6.3.2.2, Gamma-ECS, GCS heavy chain, GLCL, GLCLC

Gene Symbol

GCLC

Additional GCLC Products

Product Documents for GCLC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GCLC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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