GCLM Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33405
![Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405]](https://resources.bio-techne.com/images/products/GCLM-Antibody-Immunohistochemistry-Paraffin-NBP1-33405-img0005.jpg "Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-33405]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GCLM. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GCLM Antibody
![Knockout Validated: GCLM Antibody [NBP1-33405]](https://resources.bio-techne.com/images/products/GCLM-Antibody-Western-Blot-NBP1-33405-img0008.jpg "Knockout Validated: GCLM Antibody [NBP1-33405]")
Knockout Validated: GCLM Antibody [NBP1-33405]
Knockout Validated: GCLM Antibody [NBP1-33405] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with GCLM antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: GCLM Antibody [NBP1-33405]](https://resources.bio-techne.com/images/products/GCLM-Antibody-Western-Blot-NBP1-33405-img0006.jpg "Western Blot: GCLM Antibody [NBP1-33405]")
Western Blot: GCLM Antibody [NBP1-33405]
Western Blot: GCLM Antibody [NBP1-33405] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:5000.![Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-33405]](https://resources.bio-techne.com/images/products/GCLM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33405-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-33405]")
Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-33405]
Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-33405] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.Applications for GCLM Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Reviewed Applications
Read 2 reviews rated 4 using NBP1-33405 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GCLM
Alternate Names
Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCS light chain, GLCLRglutamate--cysteine ligase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD), Glutamate--cysteine ligase modifier subunit, glutamate-cysteine ligase regulatory protein, glutamate-cysteine ligase, modifier subunit, GSC light chain
Gene Symbol
GCLM
Additional GCLM Products
Product Documents for GCLM Antibody
Product Specific Notices for GCLM Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.