GCLM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83361

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-83361
NBP1-83361-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCLM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GCLM Antibody

Western Blot: GCLM Antibody [NBP1-83361]

Western Blot: GCLM Antibody [NBP1-83361]

Western Blot: GCLM Antibody [NBP1-83361] - Analysis in human cell line U-251 MG and human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-83361]

Immunocytochemistry/ Immunofluorescence: GCLM Antibody [NBP1-83361]

Immunocytochemistry/Immunofluorescence: GCLM Antibody [NBP1-83361] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-83361]

Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-83361]

Immunohistochemistry-Paraffin: GCLM Antibody [NBP1-83361] - Staining of human thyroid gland shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Applications for GCLM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCLM

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Alternate Names

Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, GCS light chain, GLCLRglutamate--cysteine ligase regulatory subunit, glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD), Glutamate--cysteine ligase modifier subunit, glutamate-cysteine ligase regulatory protein, glutamate-cysteine ligase, modifier subunit, GSC light chain

Gene Symbol

GCLM

Additional GCLM Products

Product Documents for GCLM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCLM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.