GCN2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38126
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for GCN2 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GCN2 Antibody
![Western Blot: GCN2 Antibody [NBP2-38126]](https://resources.bio-techne.com/images/products/GCN2-Antibody-Western-Blot-NBP2-38126-img0003.jpg "Western Blot: GCN2 Antibody [NBP2-38126]")
Western Blot: GCN2 Antibody [NBP2-38126]
Western Blot: GCN2 Antibody [NBP2-38126] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG![Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP2-38126]](https://resources.bio-techne.com/images/products/GCN2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38126-img0005.jpg "Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP2-38126]")
Immunocytochemistry/ Immunofluorescence: GCN2 Antibody [NBP2-38126]
Immunocytochemistry/Immunofluorescence: GCN2 Antibody [NBP2-38126] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.![Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126]](https://resources.bio-techne.com/images/products/GCN2-Antibody-Immunohistochemistry-Paraffin-NBP2-38126-img0004.jpg "Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126]")
Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126]
Immunohistochemistry-Paraffin: GCN2 Antibody [NBP2-38126] - Staining of human gallbladder shows weak cytoplasmic positivity in glandular cells.Applications for GCN2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GCN2
Long Name
General Control Nonrepressed 2-like Protein
Alternate Names
EIF2AK4
Gene Symbol
EIF2AK4
UniProt
Additional GCN2 Products
Product Documents for GCN2 Antibody
Product Specific Notices for GCN2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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