GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81274
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free [NBP2-81274]](https://resources.bio-techne.com/images/products/GCN4-Antibody-C11L34-Immunocytochemistry-Immunofluorescence-NBP2-81274-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free [NBP2-81274]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Yeast
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Surface Plasmon Resonance, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Lambda Clone # C11L34
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Biotinylated GCN4-peptide (HLENEVARLKK).
Reactivity Notes
Expected to react with Saccharomyces cerevisiae.
Specificity
This antibody binds to the HLENEVARLKK peptide from the leucine-zipper domain of the S. cerevisiae GCN4 transcriptional activator with picomolar affinity.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Lambda
Scientific Data Images for GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free [NBP2-81274]
Immunocytochemistry/Immunofluorescence: GCN4 Antibody (C11L34) [NBP2-81274] - Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody (C11L34). Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 [NBP2-81274] followed by an Alexa Fluor 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondrial DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) NBP2-81274 with Alexa Fluor® 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels. Images courtesy of Luming Chen (Kim Orth lab, UT Southwestern Medical Center).Applications for GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Surface Plasmon Resonance
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to the GCN4-leucine zipper peptide, which is part of the dimerisation- and DNA-binding domain of the transcriptional activator GCN4 in S. cerevisiae.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: GCN4
Alternate Names
AAS101, AAS3, Amino acid biosynthesis regulatory protein, amino acid starvation-responsive transcription factor GCN4, ARG9, General Control Protein 4
Gene Symbol
GCN4
UniProt
Additional GCN4 Products
Product Documents for GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free
Product Specific Notices for GCN4 Antibody (C11L34) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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