GCS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14967

Novus Biologicals, part of Bio-Techne
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NBP2-14967
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GCS1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human GCS1. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29465311).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GCS1 Antibody

Western Blot: GCS1 Antibody [NBP2-14967]

Western Blot: GCS1 Antibody [NBP2-14967]

Western Blot: GCS1 Antibody [NBP2-14967] - (30 ug whole cell lysate) H12997. 5% SDS PAGE, antibody diluted at 1:1000.
Western Blot: GCS1 Antibody [NBP2-14967]

Western Blot: GCS1 Antibody [NBP2-14967]

Western Blot: GCS1 Antibody [NBP2-14967] - analysis of GCS1 in (L1) 100 ug of WT MEF cells and (L2) MEFs treated with GCS1 specific siRNA for 48 hours using anti-GCS1 antibody. * indicates the non-specific band recognized by the antibody. Image from verified customer review.
Immunoprecipitation: GCS1 Antibody [NBP2-14967]

Immunoprecipitation: GCS1 Antibody [NBP2-14967]

Immunoprecipitation: GCS1 Antibody [NBP2-14967] - Immunoprecipitation was performed by using 3ug of [NBP2-14967] in 2 mg whole cell lysate from WT MEF cells. The precipitated proteins were enriched with Protein A beads. The eluted proteins were analyzed through 12.5% SDS PAGE. Arrow indicates GCS1 band. Image from verified customer review.

Applications for GCS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID:29465311)

Immunoprecipitation

Validated from a verified customer review.

Knockdown Validated

Reported in scientific literature (PMID:29465311)

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-14967 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GCS1

This gene encodes the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor. This protein is located in the lumen of the endoplasmic reticulum. Defects in this gene are a cause of type IIb congenital disorder of glycosylation (CDGIIb). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Mannosyl-oligosaccharide glucosidase

Alternate Names

EC 3.2.1.106, MOGS

Gene Symbol

MOGS

UniProt

Q13724 (Human)

Additional GCS1 Products

Product Documents for GCS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GCS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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