GDF-15 Antibody (6D12.H10.E4) - C-terminus
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44214
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Lambda Clone # 6D12.H10.E4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This Protein-A purified GDF-15 Antibody (6D12.H10.E4) was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative. (Uniprot: Q99988)
Reactivity Notes
A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.
Specificity
This antibody reacts with the C-terminus of endogenous GDF-15 protein from mouse tissues. A BLAST analysis suggests reactivity with GDF-15 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with GDF-15 from other sources has not been determined.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Lambda
Description
This product was purified from concentrated tissue culture supernatant Protein G chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for GDF-15 Antibody (6D12.H10.E4) - C-terminus
![Knockdown Validated: GDF-15 Antibody (6D12.H10.E4) - C-terminus [NBP2-44214]](https://resources.bio-techne.com/images/products/GDF-15-Antibody-6D12-H10-E4-Knockdown-Validated-NBP2-44214-img0002.jpg "Knockdown Validated: GDF-15 Antibody (6D12.H10.E4) - C-terminus [NBP2-44214]")
Knockdown Validated: GDF-15 Antibody (6D12.H10.E4) - C-terminus [NBP2-44214]
Knockdown Validated: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Western blot shows lysates of HepG2 human liver carcinoma parental cell line and GDF-15 knockout (KO) HepG2 cell line. PVDF membrane was probed with 1:250 of Rat Anti-Human GDF-15 Polyclonal Antibody (Catalog # NBP2-44214) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog #HAF006). Specific band was detected for GDF-15 at approximately 33 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.![Western Blot: GDF-15 Antibody (6D12.H10.E4)C-terminus [NBP2-44214]](https://resources.bio-techne.com/images/products/GDF-15-Antibody-6D12-H10-E4-Western-Blot-NBP2-44214-img0001.jpg "Western Blot: GDF-15 Antibody (6D12.H10.E4)C-terminus [NBP2-44214]")
Western Blot: GDF-15 Antibody (6D12.H10.E4)C-terminus [NBP2-44214]
Western Blot: GDF-15 Antibody (6D12.H10.E4) [NBP2-44214] - Analysis of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained. Lane 2: MBP-NAG-1 fusion protein 1. Lane 3: MBP-NAG-1 fusion protein 2. Lane 4: MBP. Load 40ng. Primary Antibody: Rat anti-Nag 1 (C terminal specific). Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed.
GDF-15 Antibody (6D12.H10.E4) - C-terminus
Western Blot of GDF-15 Antibody (6D12.H10.E4) - C-terminus. Lane 1: Opal Prestained Molecular Weight MarkerApplications for GDF-15 Antibody (6D12.H10.E4) - C-terminus
Application
Recommended Usage
ELISA
1:2000
Western Blot
1:1000
Application Notes
This Protein G This product has been tested by ELISA, SDS-PAGE, and western blot for mouse NAG-1 protein. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately ~58kDa using the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: GDF-15
GDF15 antibodies are useful for studies on inflamation and immune system research.
Long Name
Growth Differentiation Factor 15
Alternate Names
GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta
Gene Symbol
GDF15
UniProt
Additional GDF-15 Products
Product Documents for GDF-15 Antibody (6D12.H10.E4) - C-terminus
Product Specific Notices for GDF-15 Antibody (6D12.H10.E4) - C-terminus
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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