Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 7C12.B3.F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GDF-15 Antibodies »

Product Summary for GDF-15 Antibody (7C12.B3.F2)

Immunogen

This Protein A purified GDF-15 Antibody (7C12.B3.F2) was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human GDF-15 protein. A residue of cysteine was added to facilitate coupling to KLH. (Uniprot: Q99988)

Reactivity Notes

A BLAST analysis was used to suggest partial reactivity with GDF-15 from chimpanzee and macaque based on a 92% homology. Cross-reactivity with GDF-15 from other sources has not been determined.

Specificity

This antibody specifically reacts with an H variant sequence of human GDF-15 protein from human tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This product was purified from concentrated tissue culture supernatant Protein A chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for GDF-15 Antibody (7C12.B3.F2)

Western Blot: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725]

Western Blot: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725]

Western Blot: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725] - Detection of recombinant GDF-15 protein present in Pichia pastoris whole cell lysates expressed in yeast cells : lane 1 - GDF-15 H variant with SUMO expression tag at 36 kDa; lane 2 - GDF-15 D variant with SUMO expression tag at 36 kDa; lane 3 - GDF-15 H variant; and lane 4 - GDF-15 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the VersaDoc(TM) 4000MP Imaging System.
ELISA: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725]

ELISA: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725]

ELISA: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725] - ELISA Results of GDF-15 antibody (7C12.B3.F2). Each well was coated in duplicate with 0.1ug of conjugate [H-variant Red line, D-variant Green line]. The working dilution is 1:35,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Rabbit Anti-Mouse IgG HRP conjugated and TMB substrate.

Applications for GDF-15 Antibody (7C12.B3.F2)

Application
Recommended Usage

ELISA

1:150000

Western Blot

1:1000
Application Notes
This Protein A purified This product has been tested by ELISA and western blotting for human NAG-1 protein. This reagent is particularly useful to differentiate polymorphic forms of NAG-1 protein present in human serum samples. This antibody is useful in dual antibody immunometric assays (EIA). Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GDF-15

Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human)

Gene Symbol

GDF15

UniProt

Q99988

Additional GDF-15 Products

Product Documents for GDF-15 Antibody (7C12.B3.F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GDF-15 Antibody (7C12.B3.F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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