Gelsolin/GSN Antibody (5O10O1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16138

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16138-100ul
NBP3-16138-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O10O1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Gelsolin/GSN Antibodies »

Product Summary for Gelsolin/GSN Antibody (5O10O1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Gelsolin/GSN (NP_000168.1). DQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gelsolin/GSN Antibody (5O10O1)

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Western blot analysis of extracts of various cell lines, using Gelsolin/GSN Rabbit mAb (NBP3-16138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Immunohistochemistry of paraffin-embedded mouse brain using Gelsolin/GSN Rabbit mAb (NBP3-16138) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138]

Western Blot: Gelsolin/GSN Antibody (5O10O1) [NBP3-16138] - Western blot analysis of extracts of various cell lines, using Gelsolin/GSN Rabbit mAb (NBP3-16138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Applications for Gelsolin/GSN Antibody (5O10O1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody (5O10O1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Gelsolin/GSN Antibody (5O10O1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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