Gelsolin/GSN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27566

Novus Biologicals, part of Bio-Techne
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NBP2-27566
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Gelsolin/GSN Antibodies »

Product Specifications

Immunogen

Peptide with sequence PQGRVPEARPNS-C corresponding to N-Terminus according to NP_000168.1, NP_937895.1, NP_001121138.1.

Specificity

This antibody is expected to recognize isoform a (NP_000168.1) only . Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1, NP_001121136.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Gelsolin/GSN Antibody

Western Blot: Gelsolin/GSN Antibody [NBP2-27566]

Western Blot: Gelsolin/GSN Antibody [NBP2-27566]

Western Blot: Gelsolin/GSN Antibody [NBP2-27566] - (1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Gelsolin/GSN Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in human colon, duodenum, heart and skeletal muscle lysates (calculated MW of 85.7 kDa band according to NP_000168.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gelsolin/GSN

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Alternate Names

ADF, AGEL, Brevin, GSN

Gene Symbol

GSN

UniProt

NP_000168.1 (Human)

Additional Gelsolin/GSN Products

Product Documents for Gelsolin/GSN Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Gelsolin/GSN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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