Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all GEMIN2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1. The immunogen is located within the last 50 amino acids of SIP1. Amino Acid Squence: VSRYFDQRDLADEPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GEMIN2 Antibody - BSA Free

Western Blot: GEMIN2 AntibodyBSA Free [NBP1-77177]

Western Blot: GEMIN2 AntibodyBSA Free [NBP1-77177]

Western Blot: GEMIN2 Antibody [NBP1-77177] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1ug/mL.
GEMIN2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] -

Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunocytochemistry of GEMIN2 in HeLa cells with GEMIN2 antibody at 4 u/mL.
GEMIN2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] -

Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunofluorescence of GEMIN2 in HeLa cells with GEMIN2 antibody at 20 u/mL.

Applications for GEMIN2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

4-20 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GEMIN2

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

Alternate Names

Component of gems 2, gemin-2, GEMIN2survival of motor neuron protein-interacting protein 1, SIP1-delta, SMN interacting protein 1-delta, SMN-interacting protein 1, survival of motor neuron protein interacting protein 1

Entrez Gene IDs

8487 (Human)

Gene Symbol

GEMIN2

UniProt

O14893 (Human)

Additional GEMIN2 Products

Product Documents for GEMIN2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GEMIN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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