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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CPTC-GMNN-1

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human full-length protein (Uniprot: O75496)

Localization

Nuclear expression in highly proliferating cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79743).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free [NBP2-79878]

Immunocytochemistry/ Immunofluorescence: Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free [NBP2-79878]

Immunocytochemistry/Immunofluorescence: Geminin Antibody (CPTC-GMMN-1) - Azide and BSA Free [NBP2-79878] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Geminin with Geminin Antibody (CPTC-GMMN-1) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin
Protein Array: Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free [NBP2-79878]

Protein Array: Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free [NBP2-79878]

Protein Array: Geminin Antibody (CPTC-GMMN-1) - Azide and BSA Free [NBP2-79878] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Geminin Antibody (CPTC-GMMN-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Geminin

Geminin is a multifunctional nuclear protein invloved in embyro development/differentiation, cell cycle regulation and DNA replciation. Originally defined as an inhibitor of DNA replication and substrate of the anaphase promoting complex, Geminin has also been shown to expand the neural plate in the developing embryos.

Geminin prevents DNA replication by blocking the MCM complex incorporation into prereplication complex (pre-RC). It is absent during the G1 phase and accumulates during the S, G2, and M phases of mitosis. Geminin is then degraded during the metaphase-anaphase trasitio,n allowing replication to occur during subsequent cycles.

Geminin antibodies are useful tools for a variety of research including developmental embryology, stem cell differentiation, neuroscience, and DNA replication studies.

Alternate Names

Gem, geminin, geminin, DNA replication inhibitor, RP3-369A17.3

Gene Symbol

GMNN

Additional Geminin Products

Product Documents for Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Geminin Antibody (CPTC-GMNN-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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