GFP Antibody (859)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43575

Novus Biologicals, part of Bio-Techne
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NBP2-43575
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Insect - Drosophila, Jellyfish

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Sandwich ELISA, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 859

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GFP Antibodies »

Product Specifications

Immunogen

Full length GFP recombinant protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for GFP Antibody (859)

Western Blot: GFP Antibody (859) [NBP2-43575]

Western Blot: GFP Antibody (859) [NBP2-43575]

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: GFP Antibody (859) [NBP2-43575]

Immunocytochemistry/ Immunofluorescence: GFP Antibody (859) [NBP2-43575]

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GFP stained by GFP antibody [859] diluted at 1:2000.Blue: Hoechst 33342 staining.
Western Blot: GFP Antibody (859) [NBP2-43575]

Western Blot: GFP Antibody (859) [NBP2-43575]

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [859] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
View 3 more images

Applications for GFP Antibody (859)

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Immunohistochemistry

10 - 1:500

Immunohistochemistry Whole-Mount

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
Use in In vivo reported in scientific literature (PMID:34246771).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GFP

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

Long Name

Green Fluorescent Protein

Alternate Names

eGFP, GFPuv

Additional GFP Products

Product Documents for GFP Antibody (859)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GFP Antibody (859)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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