GFP Antibody

Immunocytochemistry/Immunofluorescence Staining of GFP in Transfected HEK293 Cells

HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ICC/IF image submitted by a verified customer review.

Western Blot Detection of GFP Using Alkaline Phosphatase Conjugated Antibody

Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Lane 1: GFP (50 ng). Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa for GFP.

Western Blot Detection of GFP in Transfected HeLa Cells

9 Poly-GA co-immuno - precipitation with pATM. Aggregated poly-GA selectively co-immunoprecipitates with pATM. Image collected and cropped by CiteAb from the following publication (www.link.springer.com/article/10.1007%2Fs00401-019-02082-0) licensed under a CC-BY license.

Immunohistochemical Detection of GFP in Transgenic Mouse Embryo

E5.5 Hex-GFP transgenic mouse embryo tissue. Primary antibody: Goat anti-GFP at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.

Immunofluorescent Staining of GFP in Mouse Brain

IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.

Immunohistochemical Detection of GFP in Free Floating Mouse Brain

Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS o/n, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating immunohistochemistry to detect GFP. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:500 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Immunofluorescent Staining of Human Breast Carcinoma Tissue Using DyLight 488 Conjugated GFP Antibody

Analysis of DyLight 488 conjugate of NB100-1770. Human breast carcinoma tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLight 488.

Immunohistochemical Detection of GFP in Transplanted NPCs

GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. IHC image submitted by a verified customer review.

Western Blot Detection of GFP in HeLa Cells

Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.

Use of Anti-GFP Antibody in Western Blot

Western Blot of GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Use of Anti-GFP Antibody in Multi-Lysate Western Blot

Multi-lysate Western Blot of GFP Antibody. Marker: Opal Pre-stained ladder

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in anterior DG hilar neurons impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aDG. Scale bar, 200 μm. c Representative images & quantifications of cFos immunoreactivity in granule cell layer of aDG (GFP: n = 7, Cre: n = 4). Scale bar, 75 μm. d Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 11, Cre: n = 6). e Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). f Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 11, Cre: n = 6). g Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 11, Cre: n = 6). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: GFP Antibody [NB100-1770] -

Immunohistochemistry: GFP Antibody [NB100-1770] - Macrophages regulate dormancy in tumor cells.a Representative image of triple immunofluorescently stained in E0771-GFP primary tumor tissue for tumor cells, macrophages, & NR2F1. Green = GFP; Red = NR2F1; White = IBA-1; Blue = DAPI. White arrow shows a macrophage. The yellow arrow contact between an NR2F1-positive tumor cell & a macrophage. Mϕ=Macrophage. Scale bar=20 μm. b Quantification showing frequency of distances between NR2F1+ tumor cells to nearest macrophage in primary tumor. Data is normalized to frequency of distances between all DAPI+ nuclei to nearest TMEM. Bar = mean. Error bars = ±SEM. n = 34 fields of view (551 × 316 µm2) in 4 animals. For comparison between 0 & 200 µm bins a two-tailed Mann-Whitney test used (p < 0.0001). ****p < 0.0001. c Representative immunofluorescence images of NR2F1 expression in E0771-GFP tumor cells cultured alone, in direct contact w/ BAC1.2F5 macrophages, or in direct contact w/ HUVEC endothelial cells. White arrows show macrophages or endothelial cells in direct contact w/ a tumor cell. Green = GFP; Red = NR2F1; Blue = DAPI. TC = Tumor Cell. Mϕ = Macrophage. EC = Endothelial Cell. Scale bar = 15 μm. d Percentage of NR2F1-positive tumor cells from each group in C. TC alone: n = 777 cells in 9 independent experiments; TC+Mϕ; n = 226 cells in 6 independent experiments, TC+EC = n = 359 cells in 4 independent experiments. Bar = mean. Error bars = ±SEM. For TC vs. TC+Mϕ (p = 0.0039), & for TC vs. TC+EC (p = 1), a two-tailed Kruskal-Wallis test w/ Dunn’s multiple comparisons adjustment used. For TC+Mϕ vs. TC+EC (0.012), a two-tailed one-way ANOVA w/ Sidak’s multiple comparison adjustment used. *p < 0.05. **p < 0.01; ns = not significant. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from following publication (https://pubmed.ncbi.nlm.nih.gov/35110548), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Viral recombination of Oxtr in aCA2/CA3distal impairs discrimination of social, but not non-social, stimuli. a Schematic illustrating viral injection & behavioral testing timeline. b Representative images of Cre & GFP virus infection in aCA2/CA3. Scale bar, 200 μm. c Behavioral schematic (top) & quantification (bottom) of single object exploration (GFP: n = 7, Cre: n = 10). d Behavioral schematic (top) & quantification (bottom) of novel objection recognition (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). e Behavioral schematic (top) & quantification (bottom) of social exploration test (GFP: n = 7, Cre: n = 10). f Behavioral schematic (top) & quantification (bottom) of social discrimination task (GFP: n = 7, Cre: n = 10). Quantifications are displayed as Habituation (trials 1–3), Test (trial 4), & discrimination ratio (trial 4). All data are displayed as mean ± SEM Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29222469), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of mature retinal ganglion cells.Dissociated retinal ganglion cells (RGCs) isolated from adult rats (a–c), mice (d–f) & zebrafish (g–i) were transduced with baculovirus (bv) encoding fGFP (rat & mice) or DsRed (zebrafish). Representative pictures of vehicle- (-) & bv -treated cultures (a,d,g) visualize transduced RGCs (GFP & DsRed, respectively) that were either co-stained with the neuronal marker betaIII-tubulin (Tub, red) at 2 days after transduction (d.a.t) for rat & mice RGCs (a,d) or identified by EGFP expression for zebrafish RGCs at 6 d.a.t. (g). The percentage of transduced RGCs was determined at 1, 2 & 3 days after transduction (d.a.t.) for rat & mice (b,e) & at 4 & 6 d.a.t. for zebrafish (h). Treatment effects compared to vehicle-treated controls: ***p < 0.001, **p < 0.01. Scale bars: 50 μm. RGC survival (c,f,i) was not affected by baculovirus application (bv) compared to vehicle-treated controls (-). (j) Delayed transduction of adult zebrafish RGCs with DsRed-bv after 4 days in culture. Scale bar: 50 μm. (k,l) Co-transduction of adult rat RGCs with fGFP-bv & DsRed-bv. The two viruses were added to retinal cultures simultaneously at half the concentration of single transductions. Representative pictures show co-transduced, fGFP- & DsRed-expressing RGCs (green & red, respectively) that were co-stained against the neuronal marker betaIII-tubulin (white) at 2 d.a.t. Scale bar: 50 μm. (k). The percentage of transduced RGCs was determined at 2 & 3 d.a.t. (l). Non-significant difference in co-tranduction efficiency compared to single transductions. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - Transduction of dorsal root ganglion neurons.Dissociated dorsal root ganglion neurons (DRG) isolated from postnatal (a–c) or adult (d–f) rats & adult mice (g–i) were transduced with fGFP-baculovirus (bv). Representative pictures of vehicle (-) & bv -treated cultures (a,d,g) show transduced, GFP-expressing neurons (green) that were co-stained with the neuronal marker betaIII-tubulin (Tub, red) at 2 days after transduction (d.a.t.). The percentage of transduced, GFP-expressing DRG neurons was determined at 1, 2 & 3 d.a.t. (b,e,h), revealing similar transduction efficiencies of 80–90% in postnatal & adult DRG neurons. Cell survival in postnatal (c) & adult (f, i) DRG cultures was not affected by bv-application compared to vehicle-treated controls (-). Scale bars: 100 μm. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep38928), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] -

Immunocytochemistry/ Immunofluorescence: GFP Antibody [NB100-1770] - hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gammaH2AX foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. b Fold change of gammaH2AX foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47–127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT & A3KO HeLa cells. d Fold change of pATM foci in HeLa WT & A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71–263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean ± SEM. *p < 0.05, **p < 0.01; one-way ANOVA & Tukey’s post-hoc test. Scale bar 10 μm Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY license. Not internally tested by Novus Biologicals.