GFP Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50034
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Concentration
1 mg/ml
Product Specifications
Immunogen
This GFP antibody was developed against recombinant Green Fluorescent Protein (GFP)
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GFP Antibody
![Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-50034]](https://resources.bio-techne.com/images/products/GFP-Antibody-Immunofluorescence-NBP2-50034-img0003.jpg "Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-50034]")
Immunocytochemistry/ Immunofluorescence: GFP Antibody [NBP2-50034]
Immunocytochemistry/Immunofluorescence: GFP Antibody [NBP2-50034] - Analysis of transfected HEK293 cells with a GFP-construct, in green, and stained with chicken pAb to GFP, NBP2-50034, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-50034 antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear golden yellow in color.
GFP Antibody [NBP2-50034] - Analysis of HEK293 cell lysates using goat pAb to GFP, dilution 1:1,000 (Green): [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein. The blot was simultaneously probed with chicken pAb to HSP60, dilution 1:10,000 (Red). The single band at 60kDa represents HSP60 protein expressed in all preparations.
Applications for GFP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
IgY purified
Formulation
50% PBS, 50% glycerol. Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GFP
References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001
2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075
3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9
Long Name
Green Fluorescent Protein
Alternate Names
eGFP, GFPuv
Additional GFP Products
Product Documents for GFP Antibody
Product Specific Notices for GFP Antibody
Chicken products cannot be exported to Canada.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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