GGT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32412

Novus Biologicals, part of Bio-Techne
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NBP1-32412
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all gamma-Glutamyltransferase 1/GGT1 Antibodies »

Product Summary for GGT1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GGT1. The exact sequence is proprietary.

Reactivity Notes

Rat (80%), Pig (85%), Bovine (82%).

Localization

Membrane, Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GGT1 Antibody

Western Blot: GGT1 Antibody [NBP1-32412]

Western Blot: GGT1 Antibody [NBP1-32412]

Western Blot: GGT1 Antibody [NBP1-32412] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract7.5% SDS-PAGE GGT1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
GGT1 Antibody

Immunohistochemistry-Paraffin: GGT1 Antibody [NBP1-32412] -

Immunohistochemistry-Paraffin: GGT1 Antibody [NBP1-32412] - GGT1 antibody detects GGT1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
GGT1 stained by GGT1 antibody (NBP1-32412) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GGT1 Antibody

Western Blot: GGT1 Antibody [NBP1-32412] -

Western Blot: GGT1 Antibody [NBP1-32412] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GGT1 antibody (NBP1-32412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for GGT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: gamma-Glutamyltransferase 1/GGT1

Human gamma-glutamyltransferase catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. This heteroduplex enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized. This gene encodes several transcript variants; studies suggest that many transcripts of this gene family may be non-functional or represent pseudogenes. The functional transcripts which have been fully characterized have been grouped and classified as type I gamma-glutamyltransferase. Complex splicing events may take place in a tissue-specific manner, resulting in marked dissimilarity in the 5' UTRs. Several 5' UTR transcript variants of the type I gene have been identified in different tissues and cancer cells.

Long Name

gamma-Glutamyltransferase 1

Alternate Names

CD224, D22S672, D22S73, GGT, GGT-1, GGTD

Gene Symbol

GGT1

Additional gamma-Glutamyltransferase 1/GGT1 Products

Product Documents for GGT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GGT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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