Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

IHC-F, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, Knockout Validated, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S39B-8

Concentration

1 mg/ml

View all GIT1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30546041).

Localization

Cytoplasm

Specificity

Detects approx 90kDa. Does not cross-react with GIT2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GIT1 Antibody (S39B-8)

Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423]

Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423]

Western Blot: GIT1 Antibody (S39B-8) [NBP2-22423] - Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:1000.
Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8 (NBP2-22423). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody (NBP2-22423) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody (S39B-8) [NBP2-22423] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm . Magnification: 60X.
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Applications for GIT1 Antibody (S39B-8)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of GIT1 Antibody was sufficient for detection of GIT1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:31908016). Use in KO reported in secitific publication PMID: 32460388

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Gene Symbol

GIT1

UniProt

Q9Z272 (Rat)

Additional GIT1 Products

Product Documents for GIT1 Antibody (S39B-8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GIT1 Antibody (S39B-8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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