GIT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85798

Novus Biologicals, part of Bio-Techne
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NBP1-85798
NBP1-85798-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GIT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GIT1 Antibody

Western Blot: GIT1 Antibody [NBP1-85798]

Western Blot: GIT1 Antibody [NBP1-85798]

Western Blot: GIT1 Antibody [NBP1-85798] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-85798]

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody [NBP1-85798]

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP1-85798] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798]

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
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Applications for GIT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GIT1

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Long Name

G Protein-Coupled Receptor Kinase Interacting ArfGAP 1

Alternate Names

ARF GAP GIT1

Gene Symbol

GIT1

Additional GIT1 Products

Product Documents for GIT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GIT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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