GLI-1 Antibody (CL12193)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15131
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (95%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL12193
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a synthetic peptide. Actual immunogen sequence is proprietary information.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for GLI-1 Antibody (CL12193)
Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -
Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -Staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin-GLI-1 Antibody (CL12191)-NBP3-15131-
Immunohistochemistry-Paraffin-GLI-1 Antibody (CL12191)-NBP3-15131- Staining of Human cerebellum shows moderate nuclear positivity in Purkinje cells, as well as neurons in molecular layer.
Immunohistochemistry-Paraffin-GLI-1 Antibody (CL12191)-NBP3-15131-
Immunohistochemistry-Paraffin-GLI-1 Antibody (CL12191)-NBP3-15131-Staining of mouse cerebellum shows strong nuclear positivity in Purkinje cells.
Applications for GLI-1 Antibody (CL12193)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2), 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-1
Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Alternate Names
GLI1
Gene Symbol
GLI1
Additional GLI-1 Products
Product Documents for GLI-1 Antibody (CL12193)
Product Specific Notices for GLI-1 Antibody (CL12193)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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