GLI-2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23602
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Predicted:
Hamster (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human GLI-2 protein (between residues 300-400) [UniProt# P10070]
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 26210874).
Localization
Nuclear and cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GLI-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Immunohistochemistry-Paraffin: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.Immunocytochemistry/ Immunofluorescence: GLI-2 Antibody - BSA Free [NBP2-23602]
Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.Applications for GLI-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:200 - 1:300
Immunohistochemistry-Frozen
reported in scientific literature (PMID 26210874)
Immunohistochemistry-Paraffin
1:200 - 1:300
Application Notes
In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 review rated 4 using NBP2-23602 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GLI-2
Long Name
GLI-Kruppel family member GLI2
Alternate Names
GLI2, THP2
Gene Symbol
GLI2
UniProt
Additional GLI-2 Products
Product Documents for GLI-2 Antibody - BSA Free
Product Specific Notices for GLI-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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