GLI-3 Antibody (2C9)
Novus Biologicals, part of Bio-Techne | Catalog # H00002737-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2C9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
GLI3 (NP_000159, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFA
Specificity
GLI3 (2C9)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for GLI-3 Antibody (2C9)
![Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01]](https://resources.bio-techne.com/images/products/GLI-3-Antibody-2C9-Immunocytochemistry-Immunofluorescence-H00002737-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01]")
Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01]
Immunocytochemistry/Immunofluorescence: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of monoclonal antibody to GLI3 on HeLa cell . Antibody concentration 10 ug/ml.![Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01]](https://resources.bio-techne.com/images/products/GLI-3-Antibody-2C9-Proximity-Ligation-Assay-H00002737-M01-img0002.jpg "Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01]")
Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01]
Proximity Ligation Assay: GLI-3 Antibody (2C9) [H00002737-M01] - Analysis of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).![ELISA: GLI-3 Antibody (2C9) [H00002737-M01]](https://resources.bio-techne.com/images/products/GLI-3-Antibody-2C9-ELISA-H00002737-M01-img0006.jpg "ELISA: GLI-3 Antibody (2C9) [H00002737-M01]")
ELISA: GLI-3 Antibody (2C9) [H00002737-M01]
ELISA: GLI-3 Antibody (2C9) [H00002737-M01] - Detection limit for recombinant GST tagged GLI3 is approximately 1ng/ml as a capture antibody.Applications for GLI-3 Antibody (2C9)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GLI-3
Long Name
GLI-Kruppel family member GLI3
Alternate Names
ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt
Entrez Gene IDs
2737 (Human)
Gene Symbol
GLI3
OMIM
146510 (Human)
UniProt
Additional GLI-3 Products
Product Documents for GLI-3 Antibody (2C9)
Product Specific Notices for GLI-3 Antibody (2C9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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