GLI-3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29627

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all GLI-3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of human Gli3 protein (between residues 50-120) [UniProt P10071]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (84%) and Chicken (85%).

Localization

Nucleus. Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

190 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLI-3 Antibody - BSA Free

Western Blot: GLI-3 AntibodyBSA Free [NBP2-29627]

Western Blot: GLI-3 AntibodyBSA Free [NBP2-29627]

Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody - BSA Free [NBP2-29627]

Immunocytochemistry/ Immunofluorescence: GLI-3 Antibody - BSA Free [NBP2-29627]

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-29627] - HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: GLI-3 Antibody - BSA Free [NBP2-29627]

Immunohistochemistry-Paraffin: GLI-3 Antibody - BSA Free [NBP2-29627]

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse liver.
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Applications for GLI-3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 26238488)

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
In Western blot, bands were observed at ~75 kDa (GLI3R) in both HeLa and MEF cell lysates while an additional band was observed ~150 kDa (GLI3A) in MEF cell lysate only. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NBP2-29627 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLI-3

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the full length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Long Name

GLI-Kruppel family member GLI3

Alternate Names

ACLS, ADD, Bph, GCPS, GLI3, PAPA1, PAPB, Pdn, PHS, PPDIV, Xt

Entrez Gene IDs

2737 (Human)

Gene Symbol

GLI3

UniProt

P10071

Additional GLI-3 Products

Product Documents for GLI-3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GLI-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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