Key Product Details

Species Reactivity

Mammal

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 03

Format

BSA Free

Concentration

1.0 mg/ml

View all GLP-1 Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(9-37)

Epitope

C-terminus of GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37)

Specificity

binds GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37). Cross-reaction with C-terminally amidated GLP-1 is 0-16% in various circumstances.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GLP1 Antibody (03) - BSA Free

Glucagon Antibody (03) - BSA Free [NBP2-21747] - BSA Free [NBP2-21747]
ELISA: GLP1 Antibody (03) - BSA Free [NBP2-21747]

ELISA: GLP1 Antibody (03) - BSA Free [NBP2-21747]

ELISA: Glucagon Antibody (03) - BSA Free [NBP2-21747] - The calibration curve of a sandwich assay for Non-amidated GLP-1 using NBP2-21747 as the capture antibody and NBP1-05180B as the biotinylated detection antibody.

Applications for GLP1 Antibody (03) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-21747 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. NBP2-21747 binds free GLP-1(1-37), GLP-1(7-37) and GLP-1(9-37) in solution. NBP2-21747 works well as a capture antibody with NBP1-05180B as a biotinylated detection antibody cross-reacting about 5% with C-terminally amidated GLP-1. NBP2-21747 can also be used as a capture antibody for detection of GLP-1 derivatives. (1) WB: NBP2-21747 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: GLP-1

GLP-1(7-36) amide is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Gene Symbol

GCG

Additional GLP-1 Products

Product Documents for GLP1 Antibody (03) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GLP1 Antibody (03) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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