Key Product Details

Species Reactivity

Human, Rat, Porcine

Applications

ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 08

Format

BSA Free

Concentration

1 mg/ml

View all Glucagon Antibodies »

Product Specifications

Immunogen

Synthetic N-terminal fragment of glucagon coupled to carrier

Epitope

The epitope lies within the N-terminal half of human glucagon

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Glucagon Antibody (08) - BSA Free

ELISA: Glucagon Antibody (08) - BSA Free [NBP2-21802]

ELISA: Glucagon Antibody (08) - BSA Free [NBP2-21802]

ELISA: Glucagon Antibody (08) - BSA Free [NBP2-21802] - Analysis using the Biotin conjugate of NBP2-21802. The calibration curve of a sandwich assay for Glucagon using NBP2-21802B as the biotinylated detection antibody and NBP2-21803 as the capture antibody.

Applications for Glucagon Antibody (08) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000
Application Notes
ELISA: NBP2-21802 binds free glucagon in solution. The biotin conjugated version of this antibody, NBP2-21802B, forms a sandwich ELISA pair with NBP2-21803 (as capture antibody) for measuring glucagon. We recommend using NBP2-21803 for any immunostaining experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glucagon

The protein encoded by the Glucagon gene is actually a preproprotein that is cleaved into four distinct mature peptides. One ofthese, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulatingglycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signallingpathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promotenutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an activeenteroglucagon. (provided by RefSeq)

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (08) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucagon Antibody (08) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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