Glucagon Antibody (09) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21803
![Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]](https://resources.bio-techne.com/images/products/Glucagon-Antibody-09-Immunocytochemistry-Immunofluorescence-NBP2-21803-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human, Mouse, Porcine
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Sandwich ELISA Capture
Cited:
Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 09
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic full length oxyntomodulin coupled to carrier.
Epitope
The epitope lies within the C-terminal region of glucagon.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32953935).
Specificity
Binds human glucagon. Cross-reacts less than 5% with oxyntomodulin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Glucagon Antibody (09) - BSA Free
Immunocytochemistry/ Immunofluorescence: Glucagon Antibody (09) - BSA Free [NBP2-21803]
Immunocytochemistry/Immunofluorescence: Glucagon Antibody (09) [NBP2-21803] - Staining in human pancreas tissueSignal is strong in alpha cells. Dilution: 1:4000![Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]](https://resources.bio-techne.com/images/products/Glucagon-Antibody-09-Immunohistochemistry-Paraffin-NBP2-21803-img0002.jpg "Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]")
Immunohistochemistry-Paraffin: Glucagon Antibody (09) - BSA Free [NBP2-21803]
Immunohistochemistry-Paraffin: Glucagon Antibody (09) [NBP2-21803] - Staining of human pancreas tissue . Alpha cells of the Langerhans islet were stained strongly. Dilution: 1:8000
Sandwich ELISA: Glucagon Antibody (09) - BSA Free [NBP2-21803] - Glucagon Antibody (09) [NBP2-21803] - The calibration curve of a sandwich assay for Glucagon using NBP2-21803 as the capture antibody and NBP2-21802B as the biotinylated detection antibody.
Applications for Glucagon Antibody (09) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:4000
Immunohistochemistry
1:8000
Immunohistochemistry-Paraffin
1:8000
Sandwich ELISA
1:100-1:2000
Application Notes
Use in Flow reported in scientific literature (PMID:33284818). ELISA: NBP2-21803 binds free glucagon in solution. NBP2-21803 when used as capture antibody forms a sandwich ELISA pair with NBP2-21802B as biotinylated detection antibody for measuring glucagon. IHC: NBP2-21803 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000. IF: NBP2-21803 was used in IF with a dilution of 1:4000.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Glucagon
Alternate Names
GCG, GRPP
Gene Symbol
GCG
Additional Glucagon Products
Product Documents for Glucagon Antibody (09) - BSA Free
Product Specific Notices for Glucagon Antibody (09) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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