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Glucagon Antibody (49) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23591

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mammal

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 49

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic GLP-1(9-18)

Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Specificity

Clone 49 binds human GLP-1(9-36)amide Based on inhibition ELISA, Clone 49 cross-reacts 3% with GLP-1(7-36)amide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Glucagon Antibody (49) - BSA Free

Glucagon Antibody (49) - BSA Free [NBP2-23591]

Applications for Glucagon Antibody (49) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-23591 binds free GLP-1(9-36)amide in solution. In a sandwich ELISA NBP2-23591 (as capture antibody) forms a pair with NBP2-23505 (as biotinylated detection antibody) in order to measure "degraded GLP-1" (the GLP-1 metabolite (GLP-1(9-36)amide)). WB: NBP2-23591 can be used in Western blotting.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glucagon

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (49) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glucagon Antibody (49) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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