Glucokinase/GCK Antibody (223)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89787

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 223

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glucokinase/GCK Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Glucokinase/GCK (Accession#: NP_000153.1; Leu2-Gln465).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Glucokinase/GCK Antibody (223)

Western Blot: Glucokinase/GCK Antibody (223) [NBP2-89787]

Western Blot: Glucokinase/GCK Antibody (223) [NBP2-89787]

Western Blot: Glucokinase/GCK Antibody (223) [NBP2-89787] - Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:92 kDa Observed band size:95 kDa

Applications for Glucokinase/GCK Antibody (223)

Application
Recommended Usage

ELISA

1:5000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucokinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose-6-phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non-insulin-dependent diabetes mellitus, maturity-onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Gene Symbol

GCK

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (223)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucokinase/GCK Antibody (223)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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