Glucokinase/GCK Antibody (4G6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37579

Novus Biologicals, part of Bio-Techne
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NBP2-37579
NBP2-37579-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G6

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Glucokinase/GCK Antibodies »

Product Summary for Glucokinase/GCK Antibody (4G6) - BSA Free

Immunogen

Purified recombinant fragment of human Glucokinase/GCK expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucokinase/GCK Antibody (4G6) - BSA Free

Western Blot: Glucokinase/GCK Antibody (4G6)BSA Free [NBP2-37579]

Western Blot: Glucokinase/GCK Antibody (4G6)BSA Free [NBP2-37579]

Western Blot: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Glucokinase/GCK Antibody (4G6)BSA Free [NBP2-37579]

Western Blot: Glucokinase/GCK Antibody (4G6)BSA Free [NBP2-37579]

Western Blot: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Analysis using GCK mAb against human GCK (AA: 1-198) recombinant protein. (Expected MW is 48.2 kDa)
ELISA: Glucokinase/GCK Antibody (4G6) - BSA Free [NBP2-37579]

ELISA: Glucokinase/GCK Antibody (4G6) - BSA Free [NBP2-37579]

ELISA: Glucokinase/GCK Antibody (4G6) [NBP2-37579] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Glucokinase/GCK Antibody (4G6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6-phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucokinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose-6-phosphate, GCK has much lower affinity for glucose and is not inhibited by the product. Consequently, GCK has a Km for glucose of approximately 7 mM, which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells. In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non-insulin-dependent diabetes mellitus, maturity-onset diabetes of the young type 2, and hyperinsulinemia of infancy. The enzyme activity was measured using a phosphatase coupled kinase assay.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Gene Symbol

GCK

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (4G6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glucokinase/GCK Antibody (4G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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