Glucokinase/GCK Antibody (OTI3E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47778

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E3

Concentration

1 mg/ml

View all Glucokinase/GCK Antibodies »

Product Summary for Glucokinase/GCK Antibody (OTI3E3)

Immunogen

Full length human recombinant protein of human GCK (NP_000153) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens glucokinase (hexokinase 4) (GCK), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glucokinase/GCK Antibody (OTI3E3)

Western Blot: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Western Blot: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Western Blot: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.
Immunocytochemistry/ Immunofluorescence: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Immunocytochemistry/ Immunofluorescence: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.
Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (OTI3E3) [NBP1-47778]

Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Human prostate tissue.
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Applications for Glucokinase/GCK Antibody (OTI3E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glucokinase/GCK

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Entrez Gene IDs

2645 (Human)

Gene Symbol

GCK

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (OTI3E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glucokinase/GCK Antibody (OTI3E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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