Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79845

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CPTC-GPI-1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Glucose 6 phosphate isomerase Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Glucose 6 phosphate isomerase protein (Uniprot: P06744)

Localization

Cytoplasm (secreted)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79711).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images

Western Blot: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1)Azide and BSA Free [NBP2-79845]

Western Blot: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1)Azide and BSA Free [NBP2-79845]

Western Blot: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Western Blot Analysis of T47D, PC3, HePG2 cell lysates using Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1).
Immunocytochemistry/ Immunofluorescence: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845]

Immunocytochemistry/ Immunofluorescence: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845]

Immunocytochemistry/Immunofluorescence: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Immunofluorescence Analysis of human MCF-7 cells labeling GPI with Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845]

Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1).
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Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Glucose 6 phosphate isomerase

Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Alternate Names

AMFGNPI, Autocrine motility factor, DKFZp686C13233, EC 5.3.1.9, glucose phosphate isomerase, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, Neuroleukin, NLKSA36, oxoisomerase, PGI, PHI, Phosphoglucose isomerase, phosphohexomutase, Phosphohexose isomerase, phosphosaccharomutase, SA-36, Sperm antigen 36, sperm antigen-36

Gene Symbol

GPI

Additional Glucose 6 phosphate isomerase Products

Product Documents

Product Datasheets

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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