Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S355-1

Concentration

1 mg/ml

View all GluR1 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1

Localization

Cell Membrane , Endoplasmic Reticulum , Cell Junction

Specificity

Detects approx. 100kDa. Does not cross-react with GluR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GluR1 Antibody (S355-1)

Western Blot: GluR1 Antibody (S355-1) [NBP2-22399]

Western Blot: GluR1 Antibody (S355-1) [NBP2-22399]

Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
Immunocytochemistry/ Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399]

Immunocytochemistry/ Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399]

Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody (NBP2-22399) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399]

Immunocytochemistry/ Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399]

Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.
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Applications for GluR1 Antibody (S355-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of GluA1/GluR1 Glutamate Receptor Antibody was sufficient for detection of GluA1/GluR1 in 20 ug of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GluR1

Glutamate Receptor 1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for Glutamate Receptor 1. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists.

Long Name

Glutamate Receptor 1

Alternate Names

GluA1, GLURA, GRIA1

Gene Symbol

GRIA1

UniProt

P19490 (Rat)

Additional GluR1 Products

Product Documents for GluR1 Antibody (S355-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GluR1 Antibody (S355-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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