GLUT10 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-490

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all GLUT10 Antibodies »

Product Specifications

Immunogen

A synthetic peptide containing residues 367-385 (ILSTAKKTKPHPRSGDPSA) of the human GLUT10 protein.

Reactivity Notes

Human and mouse. Other species have not been tested.

Specificity

Human GLUT10 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GLUT10 Antibody - BSA Free

Western Blot: GLUT10 AntibodyBSA Free [NB100-490]

Western Blot: GLUT10 AntibodyBSA Free [NB100-490]

Western Blot: GLUT10 Antibody [NB100-490] - NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using NB100-490.
Immunocytochemistry/ Immunofluorescence: GLUT10 Antibody - BSA Free [NB100-490]

Immunocytochemistry/ Immunofluorescence: GLUT10 Antibody - BSA Free [NB100-490]

Immunocytochemistry/Immunofluorescence: GLUT10 Antibody [NB100-490] - Dissected inner ear tissues from mice immersed in 20% sucrose PBS solution (pH7.4) followed by being embedded in OCT. Tissues snap frozen in liquid nitrogen & 7um sections were cut by cryostat. Cochlear sections from organ of Corti were dissected & rinsed by 0.1% Triton in PBS (pH7.4,PBST) for 30min. Sections were blocked with 5% goat serum in PBST at RT 1hr. NB100-490 was used at 1:200 to label the cochlear sections at 4C. Sections were incubated with secondary (anti-rabbit Cy3, 1:400) RT 1 hr. Slides mounted with fluoromont G and examined under a confocal microscope. Strong immunoreactivity (red) is observed in the inner and outer hair cells and basilar membrane. Spiral ganglion neurons display moderate staining.
Western Blot: GLUT10 AntibodyBSA Free [NB100-490]

Western Blot: GLUT10 AntibodyBSA Free [NB100-490]

Western Blot: GLUT10 Antibody [NB100-490] - Cochlea from embryonic mice and mice up to 1 yr old were removed and the pure membrane fraction loaded on 4-20% SDS gel. Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4C with NB100-490 at 1:500 dilution followed by incubation with secondary Ab at 1:3,000 dilution for 1h at room temp. Protein bands were visualized on x-ray film with SuperSignal west femto ECL substrate. Lane 1:10ug of protein from liver Lane 2:10ug of protein from cochlea (E18) Lane 3:10ug of protein from cochlea (P7) Lane 4:10ug of protein from cochlea (P10) Lane 5:10ug of protein from cochlea (P14) Lane 6:10ug of protein from cochlea (P17) Lane 7:10ug of protein from cochlea (P60) Lane 8:10ug of protein from cochlea (1 Year)

Applications for GLUT10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500-1:1000
Application Notes
This antibody has been tested in Western blot and immunofluorescence. No other applications have been tested. Western blot - Use at 1:500 to 1:1,000 dilution. *The investigator should determine the optimal dilution. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GLUT10

Non-insulin-dependent diabetes mellitus (NIDDM) is a multifactoral disease with both environmental and genetics causes. Genome-wide screening procedures have identified several susceptibility loci for NIDDM within the human genome. A putative sugar transporter that has been localized to human chromosome 20q12-q13.1, one of the genomic loci associated with NIDDM. Because of the strong resemblance of this novel protein to members of the mammalian facilitative glucose transporter family (GLUT), the protein is known as GLUT10 (HGMW-approved gene symbol SLC2A10). Data suggests that GLUT10 an excellent candidate for a susceptibility gene involved in NIDDM.

Alternate Names

ATS, Glucose transporter type 10, GLUT-10, GLUT10MGC126706, solute carrier family 2 (facilitated glucose transporter), member 10, solute carrier family 2, facilitated glucose transporter member 10

Gene Symbol

SLC2A10

Additional GLUT10 Products

Product Documents for GLUT10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GLUT10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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