Glut2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22218

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Glut2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human GLUT2 protein (between residues 50-150) [UniProt# P11168]

Localization

Integral membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glut2 Antibody - BSA Free

Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 AntibodyBSA Free [NBP2-22218]

Western Blot: Glut2 Antibody [NBP2-22218] - Analysis of Glut2 in total lysates of MIN6 cells using anti-Glut2 (NBP2-22218) antibody at 1/500 dilution. Image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Glut2 Antibody - BSA Free [NBP2-22218]

Immunocytochemistry/ Immunofluorescence: Glut2 Antibody - BSA Free [NBP2-22218]

Immunocytochemistry/Immunofluorescence: Glut2 Antibody [NBP2-22218] - Glut2 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry-Paraffin: Glut2 Antibody - BSA Free [NBP2-22218]

Immunohistochemistry-Paraffin: Glut2 Antibody [NBP2-22218] - Histology and immunostaining of pancreas in treated and untreated mice with STZ. (i, iv) H-E of a pancreatic islet in untreated and STZ-WT mice; (ii, v) and (iii,vi) reduction of Glut2 and insulin expression in STZ-WT mice compared to control mice. B. ( Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0105550), licensed under a CC-BY license.
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Applications for Glut2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1.0 - 2.0 ug/ml
Application Notes
This Glucose Transporter GLUT2 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed ~ 55 kDa. In ICC/IF membrane staining was observed in HepG2 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 3 reviews rated 4.3 using NBP2-22218 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut2

SLC2A2 (Solute Carrier Family 2), also known as Glucose Transporter GLUT2, is an integral plasma membrane glycoprotein found in the liver, islet beta cells, intestine, and kidney epithelium. This isoform of the glucose transporter mediates the two way transfer of glucose across the plasma membrane and is responsible for the uptake of glucose in beta cells. Mutations in SLC2A2 lead to Fanconi-Bickel syndrome (FBS), which results in hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose. Recent studies have shown that mutations in SLC2A2 can cause neonatal diabetes, and therefore may contribute to human insulin secretion (PMID: 22660720). Novel SLC2A2 mutations have also been discovered and are being investigated to determine their roles in FBS as well (PMID: 22145468).

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Gene Symbol

SLC2A2

UniProt

P14246, P11168

Additional Glut2 Products

Product Documents for Glut2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glut2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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