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Glut3 Antibody (6T3F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16340

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16340-100ul
NBP3-16340-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T3F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Glut3 Antibody (6T3F2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Glut3 (P11169). MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glut3 Antibody (6T3F2)

Western Blot: Glut3 Antibody (6T3F2) [NBP3-16340]

Western Blot: Glut3 Antibody (6T3F2) [NBP3-16340]

Western Blot: Glut3 Antibody (6T3F2) [NBP3-16340] - Western blot analysis of extracts of various cell lines, using Glut3 Rabbit mAb (NBP3-16340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: Glut3 Antibody (6T3F2) [NBP3-16340]

Immunoprecipitation: Glut3 Antibody (6T3F2) [NBP3-16340]

Immunoprecipitation: Glut3 Antibody (6T3F2) [NBP3-16340] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug Glut3 antibody (NBP3-16340). Western blot was performed from the immunoprecipitate using Glut3 antibody (NBP3-16340) at a dilition of 1:1000.

Applications for Glut3 Antibody (6T3F2)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glut3

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Long Name

Glucose Transporter Type 3

Alternate Names

SLC2A3

Gene Symbol

SLC2A3

Additional Glut3 Products

Product Documents for Glut3 Antibody (6T3F2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glut3 Antibody (6T3F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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