Glut4 Antibody (2E11NB) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44299

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E11NB

Format

BSA Free

Concentration

1.0 mg/ml

View all Glut4 Antibodies »

Product Summary for Glut4 Antibody (2E11NB) - BSA Free

Immunogen

Partial recombinant human Glut4 protein (amino acids 225-288)

Localization

Cell Membrane, ER Membrane, Perinuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Glut4 Antibody (2E11NB) - BSA Free

Western Blot: Glut4 Antibody (2E11NB)BSA Free [NBP2-44299]

Western Blot: Glut4 Antibody (2E11NB)BSA Free [NBP2-44299]

Western Blot: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of partial recombinant GLUT4 protein using GLUT4 antibody [clone 2E11NB].
Immunohistochemistry-Paraffin: Glut4 Antibody (2E11NB) - BSA Free [NBP2-44299]

Immunohistochemistry-Paraffin: Glut4 Antibody (2E11NB) - BSA Free [NBP2-44299]

Immunohistochemistry-Paraffin: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of a FFPE tissue section of normal human skeletal muscle using 1:200 dilution of Glut4 antibody (clone 2E11NB). The antibody generated a diffused staining in the blood vessels as well as the cytoplasm/sarcoplasma of skeletal muscles while the nuclei did not show any positivity for Glut4.
Western Blot: Glut4 Antibody (2E11NB)BSA Free [NBP2-44299]

Western Blot: Glut4 Antibody (2E11NB)BSA Free [NBP2-44299]

Western Blot: Glut4 Antibody (2E11NB) [NBP2-44299] - Analysis of human heart tissue lysate using 1:500 dilution of GLUT4 antibody [clone 2E11NB]. The antibody generated a single specific band of Glut4 at ~55 kDa molecular weight position.

Applications for Glut4 Antibody (2E11NB) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut4

Glucose Transporter GLUT4 (SLC2A4 or GLUT4) is an insulin-sensitive glucose transporter which facilitate insulin-stimulated glucose uptake in adipose tissue, skeletal muscle and cardiac tissues (that specifically express GLUT4). GLUT4 is a 12-transmembrane domain/ multi-pass membrane protein localized in endomembrane system as well as cytoplasm near perinuclear region, and facilitates glucose transport in the direction of glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation via TBC1D4 phosphorylation. Transgenic mice lacking or overexpressing GLUT4 respectively decrease or increase whole-body insulin sensitivity, suggesting its role in maintenance of glucose homeostasis, and accordingly, under obesity conditions, reduction of GLUT4 gene expression is directly related to insulin resistance development. Inflammatory cytokines produced by the adipose tissue including TNF-alpha, IL-6 etc have been related to reduce GLUT4 expression and defective GLUT4 is an underlying cause of NIDDM.

Alternate Names

SLC2A4

Gene Symbol

SLC2A4

OMIM

125853 (Human)

UniProt

P14672

Additional Glut4 Products

Product Documents for Glut4 Antibody (2E11NB) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glut4 Antibody (2E11NB) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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