Glut4 Antibody (3G10A3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22214

Novus Biologicals, part of Bio-Techne
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NBP2-22214
NBP2-22214-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 3G10A3

Format

BSA Free

Concentration

1 mg/ml

View all Glut4 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Glut4 (AA: 224-353) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Nucleus, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

54.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glut4 Antibody (3G10A3) - BSA Free

Western Blot: Glut4 Antibody (3G10A3)BSA Free [NBP2-22214]

Western Blot: Glut4 Antibody (3G10A3)BSA Free [NBP2-22214]

Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.
Immunocytochemistry/ Immunofluorescence: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunocytochemistry/ Immunofluorescence: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunocytochemistry/Immunofluorescence: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of HepG2 cells using Glucose Transporter GLUT4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) - BSA Free [NBP2-22214]

Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.
View 6 more images

Applications for Glut4 Antibody (3G10A3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glut4

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Alternate Names

SLC2A4

Gene Symbol

SLC2A4

Additional Glut4 Products

Product Documents for Glut4 Antibody (3G10A3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glut4 Antibody (3G10A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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