Glutamate Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68846

Novus Biologicals, part of Bio-Techne
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NBP1-68846
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Glutamate Dehydrogenase 1/GLUD1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ESEEQKRNRVRGILR corresponding to internal region according to NP_036216.2.

Specificity

This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

Marker

Mitochondria Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutamate Dehydrogenase Antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846]

Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846]

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Glutamate Dehydrogenase Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human brain (cerebellum) and mouse brain lysates and in human, mouse and rat liver lysates (calculated MW of 61.4 kDa band according to human NP_005262.1, of 61.3 kDa band according to mouse NP_032159.1 and of 61.4 kDa band according to rat NP_036702.1). IHC-P: Human cerebral cortex shows mitochondrial staining in neuronal cell bodies and axons.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutamate Dehydrogenase 1/GLUD1

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification.L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-51 M20867.1 1-51 52-3120 BC112946.1 16-3084

Alternate Names

GDH, GDH1

Entrez Gene IDs

14661 (Mouse); 24399 (Rat)

Gene Symbol

GLUD1

UniProt

NP_005262.1 (Human)

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutamate Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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