Glutamine Synthetase Antibody (GLUL/6600)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13928

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Bovine, Equine

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2bc Kappa Clone # GLUL/6600

Concentration

0.2 mg/ml

View all Glutamine Synthetase Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 50-250) of human Glutamine Synthetase protein (exact sequence is proprietary) (Uniprot: P15104)

Localization

Cytoplasm, Mitochondrion

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2bc Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14023)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Glutamine Synthetase Antibody (GLUL/6600)

Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of Y79 cell lysate using Glutamine Synthetase antibody (GLUL/6600).
Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Formalin-fixed, paraffin-embedded human stomachstained with Glutamine Synthetase antibody (GLUL/6600) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928]

Western Blot: Glutamine Synthetase Antibody (GLUL/6600) [NBP3-13928] - Western blot analysis of human liver tissue lysate using Glutamine Synthetase antibody (GLUL/6600).

Applications for Glutamine Synthetase Antibody (GLUL/6600)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

UniProt

P15104 (Human)

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (GLUL/6600)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutamine Synthetase Antibody (GLUL/6600)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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