Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70834

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1F4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Glutamine Synthetase Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

41.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

Western Blot: Glutamine Synthetase Antibody (OTI1F4)Azide and BSA Free [NBP2-70834]

Western Blot: Glutamine Synthetase Antibody (OTI1F4)Azide and BSA Free [NBP2-70834]

Western Blot: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutamine Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-G
Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834]

Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834]

Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of HepG2 cells using anti-Glutamine Synthetase mouse monoclonal antibody.
Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834]

Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834]

Immunohistochemistry: Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free [NBP2-70834] - Staining of paraffin-embedded Human endometrium tissue using anti-Glutamine Synthetase mouse monoclonal antibody.
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Applications for Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutamine Synthetase

Glutamine synthetase is part of the glutamine synthetase family. Specifically, Glutamine synthetase catalyzes the condensation of glutamate and ammonia to form glutamine, during the metabolism of nitrogen. Ammonia incorporation in animals occurs through the actions of glutamate dehydrogenase and glutamine synthase. Glutamate plays the central role in mammalian nitrogen flow, serving as both a nitrogen donor and nitrogen acceptor.

Glutamine synthetase antibodies are useful tools for studying nitrogen metabolism and regulation.

Alternate Names

cell proliferation-inducing protein 59, EC 4.1.1.15, EC 6.3.1.2, GLNSPIG59, Glutamate decarboxylase, glutamate-ammonia ligase, Glutamate--ammonia ligase, glutamate-ammonia ligase (glutamine synthase), glutamine synthase, glutamine synthetase, GS, PIG43, proliferation-inducing protein 43

Gene Symbol

GLUL

Additional Glutamine Synthetase Products

Product Documents for Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glutamine Synthetase Antibody (OTI1F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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