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Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25943

Novus Biologicals, part of Bio-Techne
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NBP3-25943-100ul
NBP3-25943-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # ARC53251

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Immunogen

Recombinant protein of human GPX1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] -

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of various lysates, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] -

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of Rat liver, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3.

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 1/GPX1

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Alternate Names

CGPx, GPX1, GSHPX1

Gene Symbol

GPX1

Additional Glutathione Peroxidase 1/GPX1 Products

Product Documents for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 1/GPX1 Antibody (ARC53251)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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