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Glutathione Peroxidase 1/GPX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26155

Novus Biologicals, part of Bio-Techne
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NBP2-26155

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QSTVYAFSARP corresponding to internal region (near N-Terminus) according to NP_032186.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26155]

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26155]

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26155] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Glutathione Peroxidase 1/GPX1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in mouse and rat kidney and liver lysates (calculated MW of 22.3 kDa band according to NP_032186.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 1/GPX1

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Alternate Names

CGPx, GPX1, GSHPX1

Entrez Gene IDs

14775 (Mouse); 24404 (Rat)

Gene Symbol

GPX1

Additional Glutathione Peroxidase 1/GPX1 Products

Product Documents for Glutathione Peroxidase 1/GPX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 1/GPX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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