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Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32390

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32390

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE40-73

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2. (Uniprot: P18283)

Localization

Cytoplasm, cytosol.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (JE40-73) [NBP3-32390]

Western Blot: Glutathione Peroxidase 2/GPX2 Antibody (JE40-73) [NBP3-32390] - Western blot analysis of Glutathione Peroxidase 2/GPX2 on different lysates with Rabbit anti-Glutathione Peroxidase 2/GPX2 antibody (NBP3-32390) at 1/1,000 dilution.

Lane 1: SW620 cell lysate
Lane 2: LoVo cell lysate
Lane 3: HT-29 cell lysate
Lane 4: AGS cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 1 minute 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 2/GPX2

Glutathione Peroxidase 2 is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.

Alternate Names

GIGPX, GPRP, GPX2

Gene Symbol

GPX2

Additional Glutathione Peroxidase 2/GPX2 Products

Product Documents for Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione Peroxidase 2/GPX2 Antibody (JE40-73)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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