Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08253

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LHM 2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Glutathione Peroxidase 4/GPX4 Antibodies »

Product Specifications

Immunogen

A 375P cells crude extract

Localization

Mitochondrion, Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07344).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free [NBP3-08253]

Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free [NBP3-08253]

Immunocytochemistry/Immunofluorescence: Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free [NBP3-08253] - Confocal immunofluorescence image of HepG2 cells stained with Glutathione Peroxidase 4/GPX4 Mouse Monoclonal Antibody (LHM 2) followed by goat anti- Mouse CF488 (green). RedDot is used to label the nuclei red.

Applications for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 4/GPX4

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.

Alternate Names

GPX4, PHGPx, snGPx

Gene Symbol

GPX4

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Glutathione Peroxidase 4/GPX4 Antibody (LHM 2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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