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Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26540

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26540-100ul
NBP3-26540-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8F6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Immunogen

A synthesized peptide derived from Human Glutathione S-Transferase pi 1/GSTP1 [UniProt P09211]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] -

Immunohistochemistry: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] - Image of Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] -

Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6) [NBP3-26540] - Positive Western Blot detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate.
All lanes: Glutathione S-Transferase pi 1/GSTP1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 24 kDa
Observed band size: 24 kDa

Applications for Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Glutathione S-Transferase pi 1/GSTP1

GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.

Alternate Names

DFN7, FAEES3, Glutathione STransferase pi 1, GSTP1

Gene Symbol

GSTP1

Additional Glutathione S-Transferase pi 1/GSTP1 Products

Product Documents for Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glutathione S-Transferase pi 1/GSTP1 Antibody (8F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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