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Glycogen phosphorylase, muscle form Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16689

Novus Biologicals, part of Bio-Techne
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NBP2-16689

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Rat, green monkey

Predicted:

Bovine (96%), Rabbit (95%), Rhesus Macaque (98%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycogen phosphorylase, muscle form. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (84%). Rat reactivity reported in scientific literature (PMID: 30660767).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glycogen phosphorylase, muscle form Antibody

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; p<0.0001), middle (b; F(7,40)=33.89; p<0.0001), and caudal (c; F(7,40)=9.43; p<0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For each VMN segment, data depict mean normalized GAD O.D. valuesS.E.M. Results are summarized in the table at bottom. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; LZ = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Human breast cancer. PYGM antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689]

Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000

Applications for Glycogen phosphorylase, muscle form Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glycogen phosphorylase, muscle form

Glycogen phosphorylase (EC 2.4.1.1) catalyzes and regulates the breakdown of glycogen to glucose-1-phosphate.[supplied by OMIM]

Long Name

Glycogen phosphorylase, muscle form

Alternate Names

EC 2.4.1.1, Myophosphorylase, PYGM

Gene Symbol

PYGM

UniProt

Additional Glycogen phosphorylase, muscle form Products

Product Documents for Glycogen phosphorylase, muscle form Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glycogen phosphorylase, muscle form Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.